What items do I need to check to diagnose soft chancre?

Chancroid is mainly transmitted directly through sexual intercourse. After infection, usually after an incubation period of 2 to 5 days, a small red papule develops on the external part. The chancroid papule quickly turns into a pustules. The pustules rupture after 2 to 3 days and become an ulcer. The ulcer is round or ovoid, and has stealth nature, with soft but irregular edges. The surrounding skin is congested. The base is granulation tissue rich in blood vessels, which is covered with purulent secretions. Patients with chancroid often feel pain at the ulcer, which intensifies by touching it. If it continues to expand big dildo , There are often 2 to 5 clusters of "satellite" ulcers around them. Without treatment, the lesions can often last for a month or two before finally healing and forming scars.
Diagnosis of soft chancre
Based on the history of sexual contact before the onset of the disease, especially the history of unclean intercourse, typical clinical manifestations and course, soft and flat papules, pustules, ulcers, and unilateral suppurative lymphadenitis after a short incubation period, a diagnosis can be made by direct microscopic examination and culture to detect Haemophilus durei DNA.
Basis for diagnosis: Barber's recommendations for the diagnosis of this disease are as follows: ① pudendal ulcer, one or more;② dark field microscope examination, Treponema pallidum was negative;③ syphilis serological test was negative;④ Smear samples were taken from the edge of the disease, and no Donovan bodies (Bacillus granulomatum) were found with Wright's staining, while short Gram-negative bacteria could be found.
The main examination items for soft chancre include the following points:
1. Microscopy: When smears are taken from open ulcers of chancroid and stained, Haemophilus dureli is easy to detect. When the unbroken lesions are punctured from an abscess or a horizontal ridge, the puncture fluid is also easy to detect and stained with smears. More typically chastity sissy , use methylene blue staining or Gram staining for 10times;100 times for observation under oil immersion. 2. Culture and inspection: It is difficult to isolate and cultivate chancre bacteria. Therefore, when taking pathological materials, attention should be paid to taking pus stored under the edge of chancre ulcer or pus sucked by puncturing the horizontal flap as a specimen, or to fully wash the ulcer with normal saline. After that, apply the specimen with a wet cotton swab of normal saline and send it to the laboratory for cultivation. Experts introduced that one of the international standard media for chancroid is GCHGS (Hammondgonococcalmedia), which is composed of Neisseria gonorrhoeae agar medium plus bovine hemoglobin, fetal bovine serum, vancomycin, cellulose, amino acids, etc.; the other is MHHb (Muller-Hinton agar, composed of Muller-Hinton agar, horse blood, vancomycin and other culture ingredients). The above two media can be used simultaneously to increase the positive rate. Colonies often form 24 to 48 hours after inoculation. The color is grayish yellow and clear, and its diameter is about 1 to 2 mm. Samples were taken from the colonies and were gram stained negative. They were pairs of Brevibacterium arranged in a chain. At the same time vibrator for women , a biochemical reaction should be carried out to identify it. The main ones are: weak positive oxidase test, negative peroxidase test, negative non-porphyrin test, positive nitrate reduction test adult toys for men , and positive alkaline phosphatase test.